5LK2

Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 300 mM KCL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976251
Spacegroup name	H 3
Unit cell lengths	107.329, 107.329, 128.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.610 - 1.600
R-factor	0.1771
Rwork	0.175
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ljz
RMSD bond length	0.006
RMSD bond angle	1.047
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.610	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.068	0.343
Number of reflections	72553
<I/σ(I)>	12.1	3.5
Completeness [%]	99.7	96.8
Redundancy	4.3	4.3
CC(1/2)	0.995	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES 6.5, 10.77%(v/v) PEG 8000, 7% (v/v) glycerol, 300 mM KCl