5LJN
Structure of the HOIP PUB domain bound to SPATA2 PIM peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 |
| Unit cell lengths | 89.040, 89.040, 53.563 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.960 - 2.701 |
| R-factor | 0.2352 |
| Rwork | 0.233 |
| R-free | 0.28050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oyk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.526 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.15) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.960 | 62.960 | 2.830 |
| High resolution limit [Å] | 2.700 | 8.960 | 2.700 |
| Rmerge | 0.080 | 0.022 | 0.710 |
| Rmeas | 0.098 | ||
| Rpim | 0.057 | ||
| Total number of observations | 31067 | ||
| Number of reflections | 11439 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 97.7 | 90.8 | 99.7 |
| Redundancy | 2.7 | 2.7 | 2.7 |
| CC(1/2) | 0.997 | 0.999 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.7-1.9 M (NH4)2SO4, 50 mM sodium cacodylate pH 6.4-7.0, 15 mM MgCl2 Protein:precipitant ratio; 1:2 200 nl drops |
