5LJM
Structure of SPATA2 PUB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2015-08-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.480, 51.140, 56.290 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 54.117 - 1.454 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | idealised helices in AMPLE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.772 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | AMPLE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.120 | 54.120 | 1.480 |
| High resolution limit [Å] | 1.450 | 7.970 | 1.450 |
| Rmerge | 0.042 | 0.038 | 0.379 |
| Number of reflections | 37750 | ||
| <I/σ(I)> | 12.1 | 38 | 2.2 |
| Completeness [%] | 90.2 | 99.8 | 81.2 |
| Redundancy | 3 | 3.3 | 2.9 |
| CC(1/2) | 0.998 | 0.990 | 0.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6 | 277 | Crystals grew at 277K over two weeks in: 20 mM MES pH 6.0, 200 mM NaCl, 4 mM DTT |
