5LJH
Crystal structure of human apo CRBP1/K40L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.531, 47.603, 77.944 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.630 - 1.520 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ljb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 1.610 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.031 | 0.468 |
| Number of reflections | 20143 | |
| <I/σ(I)> | 27.2 | 3.2 |
| Completeness [%] | 99.0 | 93.8 |
| Redundancy | 6.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 279 | 0.2 M ammonium chloride, 0.1 M sodium acetate, 0.2 mM zinc chloride, pH 5,0, 20% PEG 6000 |
