5LJG
Crystal structure of holo human CRBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 279 |
| Detector technology | PIXEL |
| Collection date | 2015-04-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.340, 48.133, 77.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.897 - 1.146 |
| R-factor | 0.1528 |
| Rwork | 0.152 |
| R-free | 0.17270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ljb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 1.210 |
| High resolution limit [Å] | 1.146 | 1.146 |
| Rmerge | 0.033 | 0.227 |
| Number of reflections | 37927 | |
| <I/σ(I)> | 24.9 | 3.6 |
| Completeness [%] | 80.6 | 22.4 |
| Redundancy | 5.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 279 | 0.2 M ammonium chloride, 0.1 M sodium acetate, 0.2 mM zinc chloride, pH 5,0, 20% PEG 6000 |
