5LJE
Crystal structure of holo human CRBP1/K40L,Q108L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91881 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.554, 47.414, 80.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.867 - 1.400 |
R-factor | 0.1937 |
Rwork | 0.192 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ljb |
RMSD bond length | 0.012 |
RMSD bond angle | 1.574 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.410 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.043 | 0.518 |
Number of reflections | 26828 | |
<I/σ(I)> | 18.3 | |
Completeness [%] | 99.6 | 98.1 |
Redundancy | 6.1 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 279 | 0.2 M ammonium chloride, 0.1 M sodium acetate, 0.2 mM zinc chloride, pH 5,0, 20% PEG 6000 |