5LJA
Structure of the E. coli MacB ABC domain (P6122)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 55.980, 55.980, 259.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.480 - 2.400 |
| R-factor | 0.22685 |
| Rwork | 0.223 |
| R-free | 0.29765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l2t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.735 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.480 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 10332 |
| <I/σ(I)> | 12.2 |
| Completeness [%] | 100.0 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 2.6 M sodium formate, 100 mM sodium acetate pH 4.6 |
