5LIR

Structure of the SALTY Sigma cross-reacting protein 27A (SCRP-27A) from Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 32 2 1
Unit cell lengths	99.662, 99.662, 52.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.860 - 1.750
R-factor	0.16702
Rwork	0.166
R-free	0.18666
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhq
RMSD bond length	0.023
RMSD bond angle	2.103
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	XDS (Oct 15, 2015)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.860	1.800
High resolution limit [Å]	1.750	1.750
Rmerge	0.102	1.700
Number of reflections	30563
<I/σ(I)>	11.65	0.98
Completeness [%]	100.0	99
Redundancy	8.4	8.5
CC(1/2)	0.999	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium acetate pH 4.6 40 % PEG 200