5LIO
Lysozyme, collected at rotation 360 degree per second
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.370, 78.370, 37.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.420 - 1.496 |
| R-factor | 0.19721 |
| Rwork | 0.195 |
| R-free | 0.24878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.926 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.420 | |
| High resolution limit [Å] | 1.496 | 1.496 |
| Rmeas | 0.083 | |
| Number of reflections | 18979 | |
| <I/σ(I)> | 20.06 | |
| Completeness [%] | 99.5 | |
| Redundancy | 13.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% w/v Polyethylene glycol monomethyl ether 5,000, 1.0 M Sodium chloride, 50 mM Sodium acetate trihydrate pH 4.5 |
