5LIN
Lysozyme, collected at rotation 1 degree per second
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.977, 77.977, 36.949 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.140 - 1.496 |
R-factor | 0.1844 |
Rwork | 0.182 |
R-free | 0.23343 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dpx |
RMSD bond length | 0.021 |
RMSD bond angle | 2.054 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.140 | |
High resolution limit [Å] | 1.496 | 1.496 |
Rmeas | 0.069 | |
Number of reflections | 18845 | |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.5 | |
Redundancy | 13.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% w/v Polyethylene glycol monomethyl ether 5,000, 1.0 M Sodium chloride, 50 mM Sodium acetate trihydrate pH 4.5 |