5LHM
Crystal Structure of SafC from Myxococcus xanthus apo-Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-31 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.558, 64.558, 118.163 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.180 - 1.310 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hnk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.160 | 1.380 |
| High resolution limit [Å] | 1.310 | 1.311 |
| Rmerge | 0.144 | 1.274 |
| Number of reflections | 69174 | |
| <I/σ(I)> | 12.6 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.2 | 20.3 |
| CC(1/2) | 0.994 | 0.405 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG MME 5000, Sodium Acetate pH 5.5 |
