5LHM

Crystal Structure of SafC from Myxococcus xanthus apo-Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-31
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999990
Spacegroup name	P 31 2 1
Unit cell lengths	64.558, 64.558, 118.163
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.180 - 1.310
R-factor	0.178
Rwork	0.177
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hnk
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	MOLREP
Refinement software	BUSTER-TNT (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	118.160	1.380
High resolution limit [Å]	1.310	1.311
Rmerge	0.144	1.274
Number of reflections	69174
<I/σ(I)>	12.6	2.3
Completeness [%]	100.0	100
Redundancy	20.2	20.3
CC(1/2)	0.994	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	PEG MME 5000, Sodium Acetate pH 5.5