5LHL
Crystal Structure of Two-Domain Laccase from Streptomyces griseoflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.640, 94.720, 117.400 |
| Unit cell angles | 90.00, 90.67, 90.00 |
Refinement procedure
| Resolution | 47.360 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cg8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.876 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 118419 |
| <I/σ(I)> | 9.09 |
| Completeness [%] | 99.5 |
| Redundancy | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.3M NaCl, 0.01 M Tris-HCl 27,5 % (w/v) PEG 4K |
