5LHJ
Bottromycin maturation enzyme BotP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 152.420, 152.420, 100.896 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.220 - 1.760 |
| R-factor | 0.17713 |
| Rwork | 0.176 |
| R-free | 0.19130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.301 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.220 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.067 | 0.543 |
| Number of reflections | 68447 | |
| <I/σ(I)> | 17 | 3.3 |
| Completeness [%] | 100.0 | 99.96 |
| Redundancy | 9.3 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350 (20 % (w/v)) and sodium malonate pH 7.0 (0.24 M) |
