5LH3
High dose Thaumatin - 0-40 ms.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 296 |
| Detector technology | PIXEL |
| Collection date | 2015-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.430, 58.430, 151.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 1.640 |
| R-factor | 0.1937 |
| Rwork | 0.193 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lr2 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.289 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.149 | 0.628 |
| Number of reflections | 30991 | |
| <I/σ(I)> | 5.6 | 10.69 |
| Completeness [%] | 92.0 | |
| Redundancy | 3.16 | |
| CC(1/2) | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.3 M sodium tartrate and 50 mM Tris, pH 6.8 |
