5LGH

Afamin antibody fragment, N14 Fab, L1- glycosilated, crystal form II, same as 5L7X, but isomorphous setting indexed same as 5L88, 5L9D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	72.196, 67.491, 88.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.060 - 1.860
R-factor	0.192
Rwork	0.190
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1il1
RMSD bond length	0.011
RMSD bond angle	1.501
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.470	1.930
High resolution limit [Å]	1.860	1.860
Rmerge	0.074	0.934
Number of reflections	36491
<I/σ(I)>	8.8	1
Completeness [%]	98.6	91.3
Redundancy	3.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	200 NL FAB (10MG/ML IN 20 MM HEPES PH 7.5, 150 MM NACL) PLUS 200 NL COCKTAIL (30% PEG 1K, 200MM AMMONIUM FLUORIDE, NH4F, pH 5.5), VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K