5LG2
Horse L type ferritin iron loaded for 60 minutes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.722 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.350, 181.350, 181.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.610 - 2.220 |
| R-factor | 0.16752 |
| Rwork | 0.165 |
| R-free | 0.22388 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f32 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.625 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.610 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.120 | 0.380 |
| Number of reflections | 13146 | |
| <I/σ(I)> | 12.6 | 4.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 297 | 80 mM Cd(NO3)2, 80 mM ammonium sulfate, and 200 mM sodium acetate pH 5 |
