5LFZ

T48 deacetylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	39.090, 56.770, 76.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.843 - 1.561
R-factor	0.1806
Rwork	0.178
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c1g
RMSD bond length	0.005
RMSD bond angle	0.957
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	1.560
Rmerge	0.049
Number of reflections	25036
<I/σ(I)>	17.3
Completeness [%]	99.7
Redundancy	6.2
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES pH 6.5 15-30% PEG (1.5K; 2K; 3350 or 4K)