5LFQ
Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis (space group P3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 3 |
| Unit cell lengths | 100.854, 100.854, 207.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.350 - 3.503 |
| R-factor | 0.2469 |
| Rwork | 0.242 |
| R-free | 0.30130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lfp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.707 |
| Data reduction software | XDS (May 1, 2016) |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.350 | 3.549 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.089 | 0.526 |
| Number of reflections | 47926 | |
| <I/σ(I)> | 8.6 | 1.41 |
| Completeness [%] | 81.4 | 78 |
| Redundancy | 2.9 | 2.4 |
| CC(1/2) | 0.997 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 5-7 % (v/v) propane-1,2-diol, 100 mM HEPES-NaOH pH 8.0 - 8.2, 200 mM MgCl2 |
