5LFM
MamA RS-1 ArsTM double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 4 |
| Unit cell lengths | 150.122, 150.122, 203.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 120.900 - 3.280 |
| R-factor | 0.17211 |
| Rwork | 0.171 |
| R-free | 0.18833 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 120.900 |
| High resolution limit [Å] | 3.280 |
| Number of reflections | 32767 |
| <I/σ(I)> | 19.41 |
| Completeness [%] | 100.0 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.8 M sodium acetate |
