5LFL

MamA RS-1 ArsTM double mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.939
Spacegroup name	I 4
Unit cell lengths	150.548, 150.548, 204.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.920 - 3.370
R-factor	0.1972
Rwork	0.196
R-free	0.21397
RMSD bond length	0.012
RMSD bond angle	1.530
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.920
High resolution limit [Å]	3.370
Number of reflections	30293
<I/σ(I)>	24.09
Completeness [%]	100.0
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2.8 M sodium acetate