5LFK

Crystal structure of CpxAHDC (hemiphosphorylated form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97934
Spacegroup name	P 61 2 2
Unit cell lengths	145.734, 145.734, 225.191
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.703 - 3.094
R-factor	0.1961
Rwork	0.195
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4biw
RMSD bond length	0.018
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	3.310
High resolution limit [Å]	3.090	3.090
Rmerge	0.094	0.774
Number of reflections	26453
<I/σ(I)>	9.4	1.8
Completeness [%]	99.6	98.1
Redundancy	11.9	11.8
CC(1/2)	0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	100 mM Tris (pH 8.5), 1.5 M ammonium sulphate and 12 % v/v glycerol