5LF2

Crystal structure of laminin beta2 LE5-LF-LE6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.920
Spacegroup name	C 1 2 1
Unit cell lengths	144.320, 55.350, 111.340
Unit cell angles	90.00, 119.38, 90.00

Refinement procedure

Resolution	62.879 - 1.850
R-factor	0.1793
Rwork	0.178
R-free	0.21020
Structure solution method	MIR
RMSD bond length	0.014
RMSD bond angle	1.280
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.900	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.057	1.290
Number of reflections	65011
<I/σ(I)>	15.7
Completeness [%]	99.1	99.2
Redundancy	6.8	7.1
CC(1/2)	0.999	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	282	Condition H4 of the Morpheus screen (Molecular Dimensions)