5LEW
DNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96864 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 256.044, 256.044, 187.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 143.110 - 2.800 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hnh |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.139 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.090 | 49.090 | 2.880 |
| High resolution limit [Å] | 2.800 | 12.200 | 2.800 |
| Rmerge | 0.207 | 0.054 | 1.501 |
| Rmeas | 0.244 | 0.067 | 1.765 |
| Rpim | 0.129 | 0.039 | 0.919 |
| Total number of observations | 197467 | 2250 | 15577 |
| Number of reflections | 57577 | ||
| <I/σ(I)> | 6.2 | 17.1 | 1.2 |
| Completeness [%] | 99.7 | 97.7 | 99.7 |
| Redundancy | 3.4 | 3.1 | 3.5 |
| CC(1/2) | 0.975 | 0.986 | 0.315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2M lithium sulphate 0.1M HEPES pH 7.5 |
