5LEV

Crystal structure of human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) (V264G mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 65 2 2
Unit cell lengths	103.250, 103.250, 239.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.710 - 3.200
R-factor	0.2347
Rwork	0.234
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j72
RMSD bond length	0.009
RMSD bond angle	0.990
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER (2.5.7)
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.790	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.084	2.360
Number of reflections	13206
<I/σ(I)>	18.2
Completeness [%]	100.0	100
Redundancy	12.6	13.3
CC(1/2)	0.999	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1M bicine pH 9.0, 0.05M sodium chloride, 38% PEG300, 5mM Na2WO4