5LED
Crystal structure of DARPin-DARPin rigid fusion, variant DDD_D12_12_D12_12_D12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.230, 47.550, 82.480 |
| Unit cell angles | 90.00, 92.04, 90.00 |
Refinement procedure
| Resolution | 47.806 - 2.600 |
| R-factor | 0.2571 |
| Rwork | 0.255 |
| R-free | 0.28980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SVX chain A |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.430 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2400)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.806 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.255 | 2.693 |
| Number of reflections | 14376 | |
| <I/σ(I)> | 18.38 | |
| Completeness [%] | 97.9 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000 20% w/v, Tris (HOAc) 0.1 M, Cadmium chloride 5 mM, pH 8.5 |
