5LE8

Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_15_D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	54.760, 73.540, 77.980
Unit cell angles	96.03, 103.43, 89.52

Refinement procedure

Resolution	43.390 - 1.780
R-factor	0.1818
Rwork	0.180
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.005
RMSD bond angle	0.721
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2405:))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.390	1.844
High resolution limit [Å]	1.780	1.780
Rmerge	0.081	2.774
Number of reflections	109030
<I/σ(I)>	8.62
Completeness [%]	95.4
Redundancy	3.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG 3350 20% w/v, Sodium citrate 0.2 M, Bis-Tris propane 0.1 M, pH 8.5