5LE6
Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_09_D12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 145.120, 145.120, 376.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.260 - 1.800 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SVX chain A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.727 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2429)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.260 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.217 | 4.336 |
| Number of reflections | 215348 | |
| <I/σ(I)> | 19.99 | |
| Completeness [%] | 99.9 | |
| Redundancy | 38.9 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 3350 17% w/v, Na2SO4 0.2 M |
