5LE4

Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_11_D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	54.290, 57.080, 147.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.727 - 2.350
R-factor	0.2342
Rwork	0.232
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.002
RMSD bond angle	0.464
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.727	2.434
High resolution limit [Å]	2.350	2.350
Rmerge	0.104	5.309
Number of reflections	19812
<I/σ(I)>	14.16
Completeness [%]	99.7
Redundancy	12.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 3350 18% w/v, KSCN 0.15 M, Tris (HOAc) 0.1 M, pH 7.5