5LDZ

Quadruple space group ambiguity due to rotational and translational non-crystallographic symmetry in human liver fructose-1,6-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	121.519, 121.519, 316.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.800 - 2.200
R-factor	0.208
Rwork	0.206
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
Data reduction software	DENZO
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.800	2.300
High resolution limit [Å]	2.200	2.200
Rmerge		1.220
Number of reflections	121069
<I/σ(I)>	7	1.1
Completeness [%]	99.7	97.9
Redundancy	7.5	4.4
CC(1/2)	0.997	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.1 M Tris-HCl pH 8.5, 2 M (NH4)2SO4