5LDU
Recombinant high-redox potential laccase from Basidiomycete Trametes hirsuta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 65 |
| Unit cell lengths | 160.650, 160.650, 53.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.16641 |
| Rwork | 0.164 |
| R-free | 0.20950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fpx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.897 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.243 |
| Number of reflections | 34900 | |
| <I/σ(I)> | 18.8 | 5.5 |
| Completeness [%] | 98.5 | 91.6 |
| Redundancy | 1.02 | 1.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.0 and 15% PEG 8,000. |
