5LDT
Crystal Structures of MOMP from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-20 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.360, 99.330, 172.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.720 - 2.880 |
| R-factor | 0.23931 |
| Rwork | 0.238 |
| R-free | 0.27192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ldv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.607 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.720 | 2.900 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.140 | 0.700 |
| Number of reflections | 36693 | |
| <I/σ(I)> | 12.9 | 3 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295.15 | 0.05M calcium chloride, 0.05M barium chloride, 0.1M Tris pH 7.5, 30% (v/v) PEG 400 |
