5LD9
Structure of deubiquitinating enzyme homolog, Pyrococcus furiosus JAMM1.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28316, 1.28375, 1.28482 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.202, 37.147, 60.131 |
| Unit cell angles | 97.29, 104.18, 100.07 |
Refinement procedure
| Resolution | 25.388 - 1.733 |
| R-factor | 0.1583 |
| Rwork | 0.157 |
| R-free | 0.18860 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.738 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.990 | 1.840 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.045 | 0.311 |
| Number of reflections | 27124 | |
| <I/σ(I)> | 12.5 | 2.7 |
| Completeness [%] | 94.0 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 65% methyl-2,4-pentanediol, 100 mM HEPES |
