5LD0
Chimeric GST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8120 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.500, 90.900, 82.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.277 - 1.600 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pkz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.898 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 28561 | |
| <I/σ(I)> | 16.95 | 1.4 |
| Completeness [%] | 96.6 | 81.3 |
| Redundancy | 3.7 | 3 |
| CC(1/2) | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | Sodium formate 0.15 M, PEG 4000 15% (w/v) |
