5LCM

STRUCTURE OF the RAD14 DNA-binding domain IN COMPLEX WITH N2-acetylaminonaphtyl- GUANINE CONTAINING DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9726
Spacegroup name	P 41
Unit cell lengths	53.041, 53.041, 131.345
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.200 - 1.900
R-factor	0.23296
Rwork	0.231
R-free	0.26868
RMSD bond length	0.018
RMSD bond angle	2.184
Data reduction software	XDS
Phasing software	REFMAC (5.8.0151)
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.148	0.800
Number of reflections	28349
<I/σ(I)>	6.2	1.1
Completeness [%]	99.0	99
Redundancy	4.5	4.5
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.2M Ammonium nitrate 40% (v/v) 2-methyl-1,3,-propanediol