5LC9
Structure of Polyphosphate Kinase from Meiothermus ruber Apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 164.783, 164.783, 94.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 116.520 - 1.903 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.19250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rhf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.910 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.520 | 2.010 |
| High resolution limit [Å] | 1.903 | 1.903 |
| Rmerge | 0.247 | 2.931 |
| Number of reflections | 100534 | |
| <I/σ(I)> | 19.8 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 52.7 | 46.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100mM TRIS/HCL pH 8.5 27%(w/v) PEG 3350 200mM Lithium sulphate |
