5LC4
Xray structure of mouse FAM3C ILEI dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.750, 118.450, 85.420 |
| Unit cell angles | 90.00, 98.78, 90.00 |
Refinement procedure
| Resolution | 48.480 - 1.840 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yop |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 1.890 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.078 | |
| Number of reflections | 69589 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | ILEI dimer 10 mg/mL + 3.3 M (NH4)2SO4, 1% MPD, 100 mM MES pH 6.5 |
