5LBK

Crystal structure of the N-domain of HMA8, a copper-transporting P-type ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-14
Detector	MARRESEARCH
Wavelength(s)	0.953
Spacegroup name	P 21 21 21
Unit cell lengths	44.170, 48.280, 108.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.031 - 1.750
R-factor	0.2001
Rwork	0.198
R-free	0.23760
RMSD bond length	0.013
RMSD bond angle	1.103
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.220	1.860
High resolution limit [Å]	1.750	1.750
Rmerge	0.065	1.102
Number of reflections	23737
<I/σ(I)>	16.5	2.64
Completeness [%]	99.4	99
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	293	0.1 M sodium acetate trihydrate pH 4.4, 0.6 M sodium formate