5LBD
Crystal structure of the N-domain of HMA6, a copper-transporting P-type ATPase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 38.800, 65.191, 85.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.960 - 1.500 |
| R-factor | 0.1849 |
| Rwork | 0.184 |
| R-free | 0.20700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | 1.101 |
| Number of reflections | 35433 | |
| <I/σ(I)> | 13.6 | 1.5 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 0.1 M sodium acetate trihydrate pH 4.4, 0.6 M sodium formate After 4 h the wells were reopened and microseeds were added |
