5LB7

Complex structure between p60N/p80C katanin and a peptide derived from ASPM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9794
Spacegroup name	C 2 2 2
Unit cell lengths	104.090, 145.120, 37.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.868 - 1.500
R-factor	0.1839
Rwork	0.183
R-free	0.22260
RMSD bond length	0.003
RMSD bond angle	0.524
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2420: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Number of reflections	45112
<I/σ(I)>	19.8
Completeness [%]	97.7
Redundancy	24.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	20% PEG 3350, 0.1 M BisTris propane, 0.2 M NaNO3