5LAE
Crystal structure of murine N1-acetylpolyamine oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972 |
Spacegroup name | P 61 |
Unit cell lengths | 121.975, 121.975, 55.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.000 - 1.850 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bq5 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.109 | 0.870 |
Number of reflections | 40245 | |
<I/σ(I)> | 14.9 | 1.9 |
Completeness [%] | 99.2 | 95 |
Redundancy | 9.8 | 6.5 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Protein at 23mg/ml in 20 mM HEPES, pH 7.0, 100 mM NaCl, 5% (v/v) glycerol and 2.5 mM TCEP was equilibrated against 2.2 M (NH4)2SO4, 0.2 M NaSCN, 0.1 M Tris pH 8 |