5LA0
The mechanism by which arabinoxylanases can recognise highly decorated xylans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.092, 72.366, 109.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.850 - 1.650 |
| R-factor | 0.12459 |
| Rwork | 0.122 |
| R-free | 0.16975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5AK1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.850 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.067 | 0.651 |
| Number of reflections | 63523 | |
| <I/σ(I)> | 13 | 1.6 |
| Completeness [%] | 98.5 | 99.4 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 1 M ammonium sulphate, 0.1 M Bis- Tris, 1 % PEG 3350 pH 5.5 |
