5L9F
S. enterica HisA mutant - D10G, G11D, dup13-15, G44E, G102A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2013-04-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.215, 91.088, 117.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.480 - 2.594 |
| R-factor | 0.2254 |
| Rwork | 0.222 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g1t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.710 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 17013 | |
| <I/σ(I)> | 9 | 2.7 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 4.8 | 4.8 |
| CC(1/2) | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 0.2 M lithium sulfate, 0.1 M sodium acetate pH4.5, 30 % w/v PEG 8000 |
