5L8X
X-RAY STRUCTURE OF APO METHANOCALDOCOCCUS JANNASCHII METHYLTRANSFERASE SUBUNIT A AT 1.85 ANGSTROM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.690, 37.030, 65.160 |
| Unit cell angles | 90.00, 91.98, 90.00 |
Refinement procedure
| Resolution | 45.108 - 1.850 |
| R-factor | 0.1894 |
| Rwork | 0.187 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MtrA cytoplasmic Methanothermus fervidus |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.110 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.123 | 0.609 |
| Number of reflections | 25433 | |
| <I/σ(I)> | 6.1 | 2 |
| Completeness [%] | 95.4 | 95.6 |
| Redundancy | 2.3 | 2.3 |
| CC(1/2) | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 281.15 | 50 mg/ml protein after 2 months incubation, transparent needle-type crystals were reproducibly obtained in 2.2 M D/L-malate pH 7.6, 100 mM Tris-HCl pH 8.0 |
