5L7S
Crystal structure of RXLR effector PexRD54 from Phytophthora infestans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 2 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 89.160, 89.160, 144.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.210 - 2.900 |
| R-factor | 0.23126 |
| Rwork | 0.230 |
| R-free | 0.25649 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.047 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.210 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 14463 | |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 18% PEG 10K, 0.1M sodium acetate pH 5.0, 0.18M tri-ammonium citrate |
