5L7R
Crystal structure of BvGH123
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.269, 147.794, 150.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.060 - 1.850 |
| R-factor | 0.18 |
| Rwork | 0.181 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.250 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.141 | 1.930 |
| Number of reflections | 106036 | |
| <I/σ(I)> | 9.9 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.1 | 8.3 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M Bis Tris pH6.5 17-19 % PEG MME 2K |
