5L7N
Plexin A1 extracellular fragment, domains 7-10 (IPT3-IPT6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.880, 82.510, 125.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.800 - 2.200 |
| R-factor | 0.2317 |
| Rwork | 0.230 |
| R-free | 0.27020 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.519 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.800 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 0.825 |
| Number of reflections | 20394 | |
| <I/σ(I)> | 9.2 | 1.9 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 8.1 | 5 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.5 | 25% (w/v) polyethylene glycol 3,350, 100mM BIS-TRIS pH 5.5 and 200mM potassium sodium tartrate |
