5L7J
Crystal Structure of Elp3 from Dehalococcoides mccartyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979230 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.460, 158.580, 93.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.294 - 2.150 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.621 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.083 | 2.309 |
| Number of reflections | 58785 | |
| <I/σ(I)> | 14.2 | 1.03 |
| Completeness [%] | 99.5 | 96.8 |
| Redundancy | 10.9 | 10.6 |
| CC(1/2) | 0.999 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM MES pH 6.3 and 4% PEG 4000 |
