5L75
A protein structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-04-10 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 105.259, 210.521, 258.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.960 - 3.700 |
| R-factor | 0.286 |
| Rwork | 0.284 |
| R-free | 0.32200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.960 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.262 | 1.700 |
| Number of reflections | 1198560 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 120 | 117.5 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 mM MES pH 6.5, 0.1 M sodium chloride, 0.1 M Lithium sulfate and 24% PEG 300 |
