5L73
MAM domain of human neuropilin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.172, 59.558, 136.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.330 - 2.240 |
| R-factor | 0.17471 |
| Rwork | 0.172 |
| R-free | 0.22827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.553 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.170 | |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 18498 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.06 M MgCl2 0.06 M CaCl2 0.1 M Tris (base), 0.1 M BICINE pH 8.0 12.5 % MPD 12.5 % PEG 1000 12.5 % w/v PEG 3350. |
