5L71
Crystal structure of mouse phospholipid hydroperoxide glutathione peroxidase 4 (GPx4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.260, 61.260, 113.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.050 - 1.800 |
| R-factor | 0.15305 |
| Rwork | 0.151 |
| R-free | 0.19264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2obi |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.463 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 23549 | |
| <I/σ(I)> | 21.6 | 4.09 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.5 | 292 | 100 mM MES, 5 mM TCEP |
